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JOHN BLACK1, MIKE WHITE2, GARY BROWN1

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1Department, Institution, Full address, Pincode, Country; 2Department, Institution, Full address, Pincode, Country; Email: john.black@gmail.com

Title of the presentation

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S. ATTA1, Y. LIU2, M. CAO2, F.Y. YANG2, Y. ZHOU2, C. ZHOU2

1Department of Biological Sciences, Karnatak University, Dharwad, Karnataka, 580003, India, Email: satta@gmail.com; 2Department of Fruit Sciences, Chinese Academy of Sciences, Beijing, 45321, China

Molecular characterization of Citrus tristeza virus isolates from India based on CPG/Hinf I restriction fragment length polymorphism (RFLP) group analysis

From six different districts of Punjab, India, 85 isolates of Citrus tristeza virus (CTV) were collected and characterized based on coat protein gene (CPG) analysis. All isolates were collected from field trees showing various CTV symptoms such as decline in most citrus varieties, inverse pitting on some sour orange rootstocks below bud union, mild-to-moderate stem-pitting on the trunk of some sweet orange. The CTV CP gene of all isolates was amplified by reverse transcriptase polymerase chain reaction (RT–PCR) using CP gene-specific primers yielding 672 bp. The maximum disease incidence was found in sweet orange followed by mandarin and grapefruit. These isolates were then subjected to CPG/Hinf I restriction fragment length polymorphism (RFLP) analysis. Mixed infection of CTV isolates was found very common in the field trees in India. The most dominant CPG/Hinf I RFLP groups III, I and VI are the basic causal epidemic in India. Moreover, based on symptoms in the field trees, CPG/Hinf I RFLP groups III, I and VI are considered to be the obvious causes of decline and stem-pitting in India.